Abstract:
Smoking tobacco is the major hazard for lung cancer in Indian subcontinent especially men, compare to
woman where, other important risk factors such as air pollutions are responsible. So, the aim of the study
is to compare chronic smokers (CS) and non-smokers living in areas with air quality categorized as poor
(AQI 201-300) or moderate (AQI 101-200). We measured the expression of non-small cell lung cancer
(NSCLC) biomarkers. IDH1, CEA, Cyfra21-1, and TPA through quantitative Real-Time PCR (qRT-PCR)
and compared the levels of upregulation of the transcripts in stage IIIa NSCLC over control benign tissues
among the smoking and AQI settings. Though the all biomarkers were significantly up-regulated in tumor
tissues compared to control benign tissues, the fold change increase of IDH1 and CEA was highest in CSpoor/moderate AQI, followed by non-smokers-poor AQI and non-smokers moderate AQI. This indicates
the aggressiveness and poor prognosis in CS living in either poor or moderate AQI areas. The level of
Cyfra21-1 was lower in in the CS groups in comparison to non-smokers in the poor AQI area. This suggest
higher Lung Squamous cell carcinoma histology in non-smokers living areas with poor AQI. Hence, we
conclude that poor air quality can be as injurious for lung cancers as chronic smoking. We also observed
pathogenic mutation KRAS p.A59T mutation comparing with CS and non-smokers under poor/moderate
AQI identified ~40% cases in all three groups suggest association with poor/moderate AQI. Additionally,
EGFR exon 20 insertion mutations in CS under poor/moderate AQI showed association with both
chronicity of smoking and poor/moderate AQI representing primary resistance to Tyrosine Kinase
Inhibitors. Hence, we conclude that poor and moderate AQI are also injurious as CS.
Biography:
She has been working to understand the specific transcriptomics-based subtyping of adult and pediatric
tumors. She has received various fellowships from Indian Government funding agencies. She has various
publications in peer reviewed journals as well has been a participant of Halifax project which wrote series of reviews (13) in Seminars of Cancer Biology and Carcinogenesis.

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