Histoplasmosis is a dangerous deep systemic mycosis that occurs in people even with preserved immune status, which can occur as respiratory distress syndrome, tuberculosis and other deseases. The importance of verification of diagnosis is associated with the vital necessity of prescribing etiotropic antimycotic therapy for patients. The causative agent of histoplasmosis is the fungus Histoplasma capsulatum which exists in two phases – the mycelial (in the external environment) and the tissue (in the macroorganism). Proof of micromycete dimorphism is the basis for laboratory diagnostics of histoplasmosis, however, there is a problem of conversion of the pathogen into the tissue (yeast) phase on nutrient media in vitro. The Francis medium is most known for this purpose, but in our studies FT-agar, intended for the cultivation of a special whimsicality to growth conditions tularemia microbe, enriched with fermented and defibrinated blood, D-glucose, sulfur-containing amino acids and vitamins complex proved to be significantly more effective. The number of colony-forming units on the FT-based Frensis medium compared with Francis medium or FT-agar without the appropriate additives in all the observations was significantly higher (p<0,01-0,001). The results obtained allow us to recommend this environment for practical use. These studies are confirmed by a Patent (RU 2 704 278 C1).
Dr. Novitskaya I. V. has graduated from Volgograd State Medical University, Russia. For a long time, she has been working at the Volgograd research anti-plague Institute as the head of the Department and an associate Professor of the Faculty of molecular biology and genetics of the medical University. Her research interests include Mycology, cell biology, biotechnology, diagnostics of dangerous and opportunistic infections. She is the author of more than 100 scientific papers, including Patents and Author’s certificates.