Wound healing consists of an organized cascade of biochemical and cellular events that involve tissue repairs and regeneration. Hibiscus sabdariffa (HS) content of polyphenols may participate by its antioxidant and anti-inflammatory properties necessary for wound healing. The purpose of this study is to assess the effect of HS loaded in ointment base applied topically to rats exposed to burning compared to standard Iruxol® ointment. Methodology: to assess wound healing potency, burn injury model was employed. Rats were divided into four groups, Group I: Normal topically applied with ointment base (Control). Group II: Burned rats were left without any treatment. Group III: Burned rats applied with topical Iruxol® ointment. Group IV: Burned rats topically applied with prepared HS extract ointment. The groups were treated with respective ointments three times through 24 hours. The wound tissues were collected to detect the oxidative stress and inflammatory effect in tissues as compared to control group. Histological analysis for tissue samples also were being endorsed the results by promoting collagen formation, re-epithelialization and angiogenesis. Results implicate that HS enhances the healing potential of the skin by stimulating the levels of biomarkers required for skin regeneration through its strong antioxidant in HS ointment applied group. In addition, HS suppressed the inflammatory effect induced by burning through its down regulation of TNF-α level. Moreover, it shows that HS topical treatment significantly reduces the hypertrophic scarring by its effect against TGF-β level. Altogether, these results suggest that HS is a valuable bioactive compound to use in wound healing and may be used with Iruxol® as synergistic product to accelerate wound processing.
Dr. Rania Khalil has her experiences in molecular biology in improving the health and protection from diseases. Her papers based on expression of genes which develop new pathways for improving healthcare. She has written these papers after several trials in research, teaching and publishing. The basis is founded on comparing gene expression before and after treatment either with a drug, complementary medicine and or food supplement. This approach has different ways of focusing that may be correlated to genetically or environmentally point of view.